Magnetic bead–based assays for the detection and measurement of 16 human Th17 biomarkers. Available in a premixed, all-in-one 15-plex assay, singleplex sets, or as custom x-Plex and Express Assays.

描述

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The Bio-Plex Pro Human Th17 Cytokine Panel enables measurement of the following analytes in diverse matrices such as serum, plasma, and cell culture supernatants:

IL-1β

IL-4

lL-6

IL-10

IL-17A

IL-17F

IL-17A/F (singleplex only)

IL-21

IL-22

IL-23

IL-25

IL-31

IL-33

IFN-γ

sCD40L

TNF-α

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammation-associatied conditions such as antimicrobial immunity, cancer, transplant rejection, and autoimmune diseases including rheumatoid arthritis, multiple sclerosis, lupus and psoriasis.

Reagent kit ll with diluents optimized for serum and plasma samples

Assay quick guide to get you started right away

Requires as little as 12.5 µl of sample for both multiplex and singleplex assays

Includes premixed standards for generation of standard curves over a broad dynamic range for each target

2-level quality controls included with premixed kits

Flexible Ordering Options

Premixed panel — complete, ready-to-use 15-plex Bio-Plex Pro Human Th17 Cytokine Panel and 3-plex Bio-Plex Pro TGF-β Assay Kits

Human Th17 singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires Reagent Kit III (171304090M) and a vial of standards (171DA0001)

Custom premixed assays (We Mix) — includes 2-level quality controls, available as Bio-Plex x-Plex Assays built using the Bio-Plex Assay Builder

Custom assays (You Mix) — available as Bio-Plex Express Assays built using the Bio-Plex Assay Builder

Additional Th17 Pathway Assays:

TGF-β 3-plex panel (171V4003M)

TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit (171304090M) for magnetic separation and a vial of standards (171D60001) for a complete assay

Instrument Compatibility

Compatible with these readers and wash stations:

Bio-Plex MAGPIX or Luminex MAGPIX System

Bio-Plex 200 or Luminex 200 System

Bio-Plex 3D or Luminex FLEXMAP 3D System

Bio-Plex Pro Wash Stations

More Information

See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

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