| Preparing The Antibody | Pack Size: 1 Conjugation For 400µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 100μg HRP you need to add between 100-400μg of antibody. For optimal results the antibody volume should be up to 100μl, at a concentration range of 0.5-5.0mg/ml.Pack Size: 3 Conjugations For 400µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 100μg HRP you need to add between 100-400μg of antibody. For optimal results the antibody volume should be up to 100μl, at a concentration range of 0.5-5.0mg/ml.Pack Size: 1 Conjugation For 4mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 1mg HRP you need to add between 1-4mg of antibody.. For optimal results the antibody volume should be up to 1ml, at a concentration range of 0.5-5.0mg/ml.Pack Size: 5 Conjugations For 4mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 4mg HRP you need to add between 1-4mg of antibody. For optimal results the antibody volume should be up to 1ml, at a concentration range of 0.5-5.0mg/ml.Pack Size: 1 Conjugation For 20mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 5mg HRP you need to add between 5-20mg of antibody. For optimal results the antibody volume should be up to 5ml, at a concentration range of 0.5-5.0mg/ml.Pack Size: 3 Conjugations For 40µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 10μg HRP you need to add between 10-40μg of antibody. For optimal results the antibody volume should be up to 10μl, at a concentration range of 0.5-5.0mg/ml. |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- Instructions For Use
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
- 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.
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