Donkey anti-Goat IgG,Alexa Fluor 647/驴抗山羊IgG,Alexa Fluor 647
货号:DG2005,DG2010
规格:50 μL,100 μL
价格:300,500
产品类型:荧光二抗
品牌:PBM
物种:山羊
宿主:驴
抗体亚型:IgG
荧光染料:Alexa Fluor 647
抗体类型: | 荧光二抗 | 同型对照: | IgG |
浓度 | 2 mg/mL | 用法: | 1-10 µg/mL(ICC/IF);1-10 µg/mL(IHC) |
产品详细信息
To minimize cross-reactivity, these donkey anti-goat IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against rabbit, rat, mouse, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Product will be shipped at Room Temperature.
靶标信息
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
数据 |
IsoLG-modified proteins are present within several lung cell populations in unstressed mice.Mouse lung tissue sections were immunostained with the following: (A) anti-SPC for Type 2 alveolar cells (Alexa 647 secondary, green false color), D11 ScFv (Rhodamine Red secondary); (B) anti T1α for Type 1 alveolar cells (Alexa 647 secondary, green false color), D11 ScFv (Rhodamine Red, secondary); (C) anti-CC10 for Club cells (Rhodamine Red secondary), D11 ScFv (Alexa 647 secondary, green false color); (D) anti-PECAM-1 for endothelial cells (Alexa 647 secondary, green false color), D11 ScFv (Rhodamine Red secondary). Images were acquired using confocal microscopy (60× magnification). The white bar represents 30 μm. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep24919), licensed under a CC BY license. IsoLG-modified proteins are present within several lung cell populations in unstressed mice.Mouse lung tissue sections were immunostained with the following: (A) anti-SPC for Type 2 alveolar cells (Alexa 647 secondary, green false color), D11 ScFv (Rhodamine Red secondary); (B) anti T1α for Type 1 alveolar cells (Alexa 647 secondary, green false color), D11 ScFv (Rhodamine Red, secondary); (C) anti-CC10 for Club cells (Rhodamine Red secondary), D11 ScFv (Alexa 647 secondary, green false color); (D) anti-PECAM-1 for endothelial cells (Alexa 647 secondary, green false color), D11 ScFv (Rhodamine Red secondary). Images were acquired using confocal microscopy (60× magnification). The white bar represents 30 μm. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep24919), licensed under a CC BY license. |
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