KLF4 Polyclonal Antibody
货号:PA5-27440
规格:100 µL
价格:5935
产品类型:干细胞鉴定
品牌:Thermo Fisher
类型: | 一抗 | 同型对照: | IgG |
浓度: | 0.23 mg/mL | 用法: | 1:50-1:200 (Flow) ;Assay Dependent (ICC);Assay Dependent (IF); 1:100-1:500(IP); 1:500-1:3,000(WB) |
产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2, Human ESC, HCT116, HCT116 nuclear extract, NIH-3T3, mouse ESC, Rat2, *MDM-MB-231, KLF4-transfected 293T.Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (86%), Cat (100%), Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Kruppel like factor 4 (KLF4) protein is encoded by the KLF4 gene located on chromosome 9 in humans. It is also known as gut-enriched Kruppel-like factor (GKLF) or epithelial zinc-finger protein (EZF) and belongs to a family of conserved Kruppel-like factor family of DNA binding zinc finger transcription factors. KLF4 exerts its functional relevance across varied cellular pathways. As one of the four Yamanaka factors, it plays a key role in reprogramming differentiated cells into pluripotent stem cells. Most studies have focused on its specific role in endothelial cell signaling, adipogenesis, differentiation, embryogenesis and cell cycle control. Localization plays an important role in the biological significance of this protein wherein nuclear localization is associated with its function as an oncogene and cytosolic localization with tumor suppression specific effect.
数据: |
KLF4 Antibody (PA5-27440) in WBWestern blot analysis of KLF4 using 30 µg of A) A549 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a KLF4 polyclonal antibody (Product # PA5-27440) at a dilution of 1:5000.KLF4 Antibody (PA5-27440) in Flow Flow cytometry analysis of KLF4 in human embryonic stem cells (purple) compared to a rabbit IgG control (black) using a KLF4 polyclonal antibody (Product # PA5-27440) at a dilution of 1:100. |
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