IL-13 Monoclonal Antibody (PVM13-1), eBioscience/IL-13单克隆抗体（PVM13-1）

货号：14-7139-81

规格：50 µg

价格：1399

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：PVM13-1

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-4 µg/mL（ELISA)；Assay-Dependent(Flow)；Assay-Dependent(FN)；Assay-Dependent(Neu)

产品详细信息Description: The PVM13-1 antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8^+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.Applications Reported: PVM13-1 has been reported for use in intracellular flow cytometric analysis, cytokine neutralization,and ELISA. (Fluorochrome conjugated PVM13-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified PVM13-1, cat. 16-7139, in functional assays.).Applications Tested: The PVM13-1 antibody has been tested as the capture antibody in a sandwich ELISA and ELISPOT assay for analysis of human interleukin-13 (IL-13) in combination with the biotinylated anti-human IL-13 polyclonal antibody (13-7138) antibody for detection and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

数据

IL-13 Antibody (14-7139-81) in ELISAHuman PBMCs (300,000/well) in 100 µL complete RPMI medium were treated without (left) or with (right) Candida (100 µg/mL) for 24 hrs in an IL-13 ELISPOT assay.

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参考文献：

1. JCI insightA molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22.2. The Journal of clinical investigationProtein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.3. Nature geneticsCOPA mutations impair ER-Golgi transport and cause hereditary autoimmune-mediated lung disease and arthritis.

技术参数

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