TNF alpha Monoclonal Antibody (TN3-19.12), FITC

货号：11-7423-82

规格：100μg

价格：1959

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

克隆号：TN3-19.12

荧光染料：FITC

类型:	单抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), FITC
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have beenobserved.Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry. Functional Grade purified TN3-19.12, cat. 16-7423, is recommended for use in functional assays. For ELISPOT capture, the alternative clone, 1F3F3D4 (cat # 16-7325), is recommended.Applications Tested: This TN3-19.12 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. It is offered in 2 formats: - µg size: can be used at less than or equal to 0.25 µg per test.- test size: can be used at 5 µL (0.125 µg) per test.A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

数据

TNF alpha Antibody (11-7423-82) in FlowMouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right), then intracellularly stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.125 µg of Anti-Mouse/Rat TNF alpha FITC. Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Neuropsychiatric disease and treatmentAnti-TNF-alpha antibody attenuates subarachnoid hemorrhage-induced apoptosis in the hypothalamus by inhibiting the activation of Erk."Published figure using TNF alpha monoclonal antibody (Product # 11-7423-82) in Western Blot"2.Nature communicationsTristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses."Published figure using TNF alpha monoclonal antibody (Product # 11-7423-82) in Neutralization"

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45297.htm