Phospho-Histone H3 (Ser28) Monoclonal Antibody (HTA28), eFluor 660

货号：50-9124-41,50-9124-42

规格：25 Tests,100 Tests

价格：0,3021

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：HTA28

荧光染料：eFluor 660

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The HTA28 monoclonal antibody recognizes phosphorylated serine 28 of human, mouse, rat, bovine, and hamster histone H3. This 15 kDa protein is a component of eukaryotic chromatin that is involved in forming the nucleosome structure. Histone H3 can be phosphorylated at serine 10 and serine 28. Studies have demonstrated that phosphorylation at serine 28 is mediated by MSK1 following activation by the MAP kinase signaling pathway in response to tumor promoters (e.g., UV and EGF) and oncoproteins (e.g., c-Myc, c-Jun, and c-Fos). Histone H3 serine 28 phosphorylation has been linked to chromosome condensation during mitosis, cell transformation, and regulation of RNA polymerase III transcription machinery.Applications Reported: This HTA28 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This HTA28 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of nocodazole-treated HeLa cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Protocols: We recommend using Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins or Protocol C: Two-step protocol: Fixation/Methanol. Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the "Staining intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据

Phospho-Histone H3 (Ser28) Antibody (50-9124-42) in FlowIntracellular staining of 21-hour nocodazole-treated HeLa cells with Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or Anti-Human/Mouse phospho-Histone H3 (S28) eFluor® 660 (purple histogram) using the IC staining protocol: Two step protocol for (cytoplasmic) intracellular proteins. Total viable cells were used for analysis.

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参考文献：

1.Disease models and mechanismsModelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture."50-9124 was used in Immunocytochemistry to explore the use of whole adult brain coronal organotypic slices as an alternative model for glioblastoma tumour-host cell interactions."2.Disease models and mechanismsModelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture."50-9124 was used in Immunocytochemistry to explore the use of whole adult brain coronal organotypic slices as an alternative model for glioblastoma tumour-host cell interactions."

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