FOXC1 Polyclonal Antibody/FOXC1多克隆抗体

货号：PA1-807

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues A(423)

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：Antibody

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1-3 µg x 10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1:500(WB)

产品详细信息PA1-807 detects FOXC1 from human and mouse samples.PA1-807 has been successfully used in Western blot procedures. By Western blot, PA1-807 detects a ~50 kDa band representing FOXC1 from HepG2 cells and mouse kidney cells. This antibody also detects a nonspecific band at ~45 kDa from HepG2 cells, and at ~60 kDa from mouse kidney samples.The PA1-807 immunogen is a synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.PA1-807 can be used with blocking peptide PEP-298.靶标信息FOXC1 is a protein belonging to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this protein is not known, however, it plays a role in the regulation of FGF19-FGFR4-MAPK pathway to promote both the development and maintenance of anterior segment structures within the eye. Mutations in this gene cause various glaucoma, iridogoniodysgenesis anomaly, Peters anomaly that includes central corneal leukoma, absenceof the posterior corneal stroma and Descemetmembrane and Axenfeld-Rieger anomaly characterized by posterior corneal embryotoxon, iris adhesion to the Schwalbe line, hypertelorism, hypoplasia of the malar bones, congenital absence of some teeth and mental retardation.

数据：

FOXC1 Antibody (PA1-807) in IFImmunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody (Product # PA1-807) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. FOXC1 Antibody (PA1-807) in ChIPEnrichment of endogenous FOXC1 Protein at specific gene loci using Anti-FOXC1 Protein Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-FOXC1 Protein Rabbit Polyclonal Antibody (Product # PA1-807, 3 µg) on sheared chromatin from 2 million HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of active FGF gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method. FOXC1 Antibody (PA1-807)Oncotarget 2018 -Published figure using FOXC1 polyclonal antibody (Product # PA1-807)

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参考文献：

1. OncotargetSystem analysis of the regulation of the immune response by CD147 and FOXC1 in cancer cell lines.2. OncotargetSystem analysis of the regulation of the immune response by CD147 and FOXC1 in cancer cell lines.

技术参数

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