Histone H3 Polyclonal Antibody/组蛋白H3多克隆抗体

货号：PA5-16183

规格：100 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the carboxy-ter

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	1:50(ChIP)；1:250(ICC)；1:250(IF)；1:1,000-1:4,000(WB)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other histones.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

Histone H3 Antibody (PA5-16183)eLife 2016 -Figure 4--figure supplement 2. Quantitation of HA-CDKG1, histone H3 and alpha-tubulin from mother cells of different sizes. ( A ) Cell size distributions of populations similar to those in Figure 4D and E used for quantitative western blotting. Lysates from Pre-commitment cells (I, median cell size ~80 um 3 ), small mother cells (II, median cell size ~350 um 3 ) and large mother cells (III, median cell size ~600um 3 ) were used for quantitation. ( B ) Anti-HA, anti-alpha tubulin, and anti-histone H3 Western blot and the corresponding Coomassie staining of total protein extracts from populations I, II and III. Gels were loaded with equal biomass per lane or with equal cell number per lane as indicated. ( C ) Signal quantitation from each experiment. The highest signal for each antibody was arbitrarily set as 1, and the other values were normalized against it. Error bars: S.D. from three technical replicates. DOI: http://dx.doi.org/ Histone H3 Antibody (PA5-16183) in ChIPChromatin immunoprecipitation of Histone H3 was performed on cross-linked chromatin from 1 x 10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 5, or 20 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1 µL/100 µL well volume of a Histone H3 polyclonal antibody (Product # PA5-16183). Chromatin aliquots from ~1 x 10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify 7kb upstream of the Egr1 gene, promoter-exon1 (transcription start site, TSS) of Egr1, or exon-2 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total rat genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by the black bars above the schematic. Data courtesy of the Innovators Program.

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参考文献：

1. Scientific reportsUncoupling of p97 ATPase activity has a dominant negative effect on protein extraction.2. Molecules (Basel, Switzerland)Modulation of Endocannabinoid-Binding Receptors in Human Neuroblastoma Cells by Tunicamycin.3. eLifeA new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division.

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