Histone H3 Recombinant Polyclonal Antibody (24 HCLC)/组蛋白H3重组多克隆抗体（24 HCLC）

货号：710546

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：proprietary

物种：人

宿主：兔

抗体亚型：IgG

克隆号：24 HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；Assay-Dependent(ELISA)；2 µg/mL(ICC)；2 µg/mL(IF)；0.5 µg/mL(WB)

产品详细信息This antibody is predicted to react with bovine, non-human primate, mouse, rat and ovine based on sequence homology.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

Histone H3 Antibody (710546) in WBWestern blot analysis of endogenous Histone H3 in 30 µg of HeLa whole cell extract treated with 5mM sodium butyrate for 24 hours (lane 1) and untreated HeLa cells (Lane 2) using a Histone H3 recombinant rabbit monoclonal antibody (Product # 710546) at a concentration of 1 µg/mL. A rabbit anti-tubulin antibody was blotted as a loading control and the blots were developed using chemiluminescence (ECL) method with a goat anti-rabbit HRP secondary antibody (Product # G-21234). Results show a band at ~17 kDa. Histone H3 Antibody (710546) in ChIPEnrichment of endogenous Histone H3 protein at specific gene loci using Anti-Histone H3 Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H3 Recombinant Rabbit Polyclonal Antibody (Product # 710546, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the promoters of MYOD, SAT2 satellite repeats used as positive, and C-FOS, GAPDH used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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