Histone H3.3 Recombinant Rabbit Monoclonal Antibody (RM190)/组蛋白H3.3重组兔单克隆抗体（RM190）

货号：MA5-24667

规格：100 µg

价格：4130

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to human Histone H3.3.

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：RM190

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；0.2-1 µg/mL(ELISA)；1 µg/mL(WB)；0.05-0.5 µg/mL(Array)

产品详细信息This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonalantibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a memberof the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

数据：

Histone H3.3 Antibody (MA5-24667) in WBWestern blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), A-549 (Lane 3), K-562 (Lane 4) and COS-7 (Lane 5). The blot was probed with Anti-Histone H3.3 Monoclonal Antibody (Product # MA5-24667, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Histone H3.3 was observed across the cell lines tested. Histone H3.3 Antibody (MA5-24667) in ChIPEnrichment of endogenous HIST2H2AB protein at specific gene loci using Anti-HIST2H2AB Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-HIST2H2AB Polyclonal Antibody (Product # PA5-48625, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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