H3K79ac Recombinant Polyclonal Antibody/H3K79ac重组多克隆抗体

货号：710805

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to Human HIST1H3A (aa 76-84)

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2-3 µg/1x10^6 cells(ChIP)；1 µg/mL(ELISA)；2µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Rat, Cat, Pig and Monkey.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonalantibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K79ac Antibody (710805) in IFFor immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous H3K79ac using H3K79ac Recombinant Rabbit Polyclonal Antibody (Product # 710805, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Localization of H3K79ac (green) in the nucleus can be clearly observed in cells treated with suberoylanilide hydroxamic acid (SAHA, 0.5 uM, 18 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the H3K79ac peptide, which results in loss of signal. No competition was observed with the non-acetylated peptide. The images were captured at 60X magnification. H3K79ac Antibody (710805) in ChIPEnrichment of endogenous Acetyl Histone H3 (Lys 79) (Histone H3K79ac) protein at specific gene loci using Anti- Histone H3K79ac Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H3K79ac Recombinant Rabbit Polyclonal Antibody (Product # 710805, 5 µg) on sheared chromatin from 2 million HeLa cells treated with 5mM sodium butyrate for 24 hours using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active CFOS, β-ACTIN region used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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