H3K79me3 Polyclonal Antibody/H3K79me3多克隆抗体

货号：49-1020

规格：50 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：raised in rabbits against histone H3 containing th

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.6 mg/mL	用法：	1-10 µg/1x10^6 cells(ChIP)；1:100,000(DB)；1:500(ELISA)；1:1,000(WB)；1 µg per assay(ChIP-Seq)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K79me3 Antibody (49-1020) in ChIPChIP assays were performed using HeLa cells, the anti-H3K79me3 antibody (Product # 49-1020), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and various amounts of anti-H3K79me3 antibody (1, 2, 5, and 10 µg). IgG (5 µg⁄IP) was used as a negative IP control. qPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. H3K79me3 shows a preference for active promoters. Therefore the promoter of GAPDH, a housekeeping gene, was used as a positive control target. Exon 2 of myoglobin was used as a negative control target. This figure shows the recovery, expressed as a percentage of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis) of the GAPDH promoter and of myoglobin exon 2. H3K79me3 Antibody (49-1020) in ChIPChIP was performed with the anti-H3K79me3 antibody (Product # 49-1020) on sheared chromatin from 1 million HeLaS3 cells. A titration of the antibody consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (5 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.

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参考文献：

1. The Journal of biological chemistryA novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression.2. The Journal of biological chemistryA novel disrupter of telomere silencing 1-like (DOT1L) interaction is required for signal transducer and activator of transcription 1 (STAT1)-activated gene expression.

技术参数

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