Phospho-Histone H3 (Ser10) Recombinant Polyclonal Antibody (9HCLC)/磷酸化组蛋白H3（Ser10）重组多克隆抗体（9HCLC）

货号：710282

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Phosoeptide corresponding to amino acids 4–16 of human Histone H3 [pS10]

物种：人

宿主：兔

抗体亚型：IgG

克隆号：9HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	3 µg(ChIP)；2-4 µg(ICC)；2-4 µg(IF)；1-3 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

Phospho-Histone H3 (Ser10) Antibody (710282) in IFImmunofluorescent analysis of Histone H3 (pS10) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Histone H3 (pS10) Recombinant Rabbit Polyclonal Antibody (Product # 710282) at 2 µg-4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization of Histone H3 (pS10). Panel e shows competition with Histone H3 (pS10) peptide. The images were captured at 20X magnification. Phospho-Histone H3 (Ser10) Antibody (710282) in ChIPChIP qPCR analysis Histone H3 (pSer10) in Hela cells using a Phospho-Histone H3 pSer10 Recombinant Rabbit Polyclonal Antibody (Product # 710282). Real-time PCR was performed for cFOS (positive locus) and SAT2 (negative locus) targets using an isotype antibody as a negative control. Data is presented as fold enrichments of the ChIP antibody signal versus the negative control IgG using the comparative CT method.

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