Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody/Phospho-SMAD2（Ser465，Ser467）多克隆抗体

货号：44-244G

规格：100 µL

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：The antiserum was produced against a chemically sy

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	10 µL(ChIP)；1:250(ICC)；1:250(IF)；1:20-1:200(IHC (P))；1:1000(WB)

靶标信息SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

数据：

Phospho-SMAD2 (Ser465, Ser467) Antibody (44-244G) in IFImmunofluorescent analysis of Phospho-SMAD2 pSer465/pSer467 Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were treated with TGF-beta1 (20 ng/mL) for 15 minutes and labeled with Phospho-SMAD2 pSer465/pSer467 Antibody (Product # 44-244G) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing Nuclear localization. Panel e showing merged image of untreated cells with less nuclear signals Panel f is a no primary antibody control. The images were captured at 40X magnification. Phospho-SMAD2 (Ser465, Ser467) Antibody (44-244G) in IHC (P)Immunohistochemistry analysis of SMAD2 (pSpS465/467) showing staining in the nucleus of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SMAD2 (pSpS465/467) polyclonal antibody (Product # 44-244G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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参考文献：

1. Cellular and molecular gastroenterology and hepatologyPancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis.2. OncoimmunologyA novel bifunctional anti-PD-L1/TGF-β Trap fusion protein (M7824) efficiently reverts mesenchymalization of human lung cancer cells.3. Genes and developmentOncogenicKrasdrives invasion and maintains metastases in colorectal cancer.4. JCI insightTherapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells.5. JCI insightTherapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells.

技术参数

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