PRMT6 Recombinant Rabbit Monoclonal Antibody (8H9L19)

货号：703547

规格：100 µg

价格：4737

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Peptides corresponding to human PRMT6 [1) aa37-aa4

物种：人

宿主：兔

抗体亚型：IgG

克隆号：8H9L19

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5µg/million cells(ChIP) ; 1:100(ICC);1:100(IF)

产品详细信息This antibody is predicted to react with Monkey, Pig, Rat, Cat.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. 靶标信息Protein arginine N-methyltransferase 6 (PRMT6) PRMT6 demonstrates type I PRMT activity, capable of forming both omega-N monomethylarginine and asymmetric dimethylarginine derivatives on the recombinant glycine- and arginine-rich substrates. cts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7.

数据：

PRMT6 Antibody (703547) in IFFor immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous PRMT6 using Anti-PRMT6 Recombinant Rabbit Monoclonal Antibody (Product # 703547, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates Nuclear and cytoplasmic localization of PRMT6 in MCF7 cells. Panel e-h) shows no specific staining in MDA-MB-231 cells which is PRMT6 negative, demonstrating specificity. The images were captured at 60X magnification. PRMT6 Antibody (703547) in ChIPEnrichment of endogenous PRMT6 protein at specific gene loci using Anti-PRMT6 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-PRMT6 Recombinant Rabbit Monoclonal Antibody (Product # 703547, 5 µg) on sheared chromatin from 2 million MCF7 cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over Promoters of CDKN1A, HXA2 (positive) and SAT Alpha (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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