AMPK alpha-1 Monoclonal Antibody (2B7)

货号：MA5-15815

规格：100 µL

价格：5628

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Purified recombinant fragment of human PRKAA1 expr

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2B7

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：
浓度：		用法：	1:200 - 1:400(Flow);1:200-1:1000(ICC)；1:200-1:1000 (IF);1:200 - 1:1000(IHC);1:1000(WB)

产品详细信息MA5-15815 targets PRKAA1 in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.The MA5-15815 immunogen is purified recombinant fragment of human PRKAA1 expressed in E. Coli.MA5-15815 detects PRKAA1 which has a predicted molecular weight of approximately 64kDa. 靶标信息Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

数据：

AMPK alpha-1 Antibody (MA5-15815) in IF Immunofluorescence analysis of AMPK alpha-1 was performed using 70% confluent log phase LNCap cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AMPK alpha-1 Monoclonal Antibody (2B7)(Product # MA5-15815) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. AMPK alpha-1 Antibody (MA5-15815) in WBKnockdown of AMPK alpha-1 was achieved by transfecting MCF7 cells with AMPK alpha-1 specific validated siRNAs (Silencer® select Product # s100, s101 ). Western blot analysis (Fig. a) was performed using whole cell extracts from the AMPK alpha-1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with AMPK alpha-1 Monoclonal Antibody (Product # MA5-15815, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to AMPK alpha-1.

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