Thermo Alexa Fluor 647 核酸标记,ARES Alexa Fluor 647 DNA Labeling Kit/Alexa Fluor 647 DNA标记试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Alexa Fluor 647 核酸标记,ARES Alexa Fluor 647 DNA Labeling Kit/Alexa Fluor 647 DNA标记试剂盒

2024-10-24

ARES Alexa Fluor 647 DNA Labeling Kit/Alexa Fluor 647 DNA标记试剂盒

货号：A21676

规格：1 kit

价格：7978

产品类型：核酸标记

品牌：Thermo Fisher

The ARES Alexa Fluor DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor 647 dye. The labeled probes can be used for fluorescencein situhybridization (FISH) and microarray techniques. We offer ARES Alexa Fluor DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5 µg DNA each.More Uniform Labeling With ARES Labeling KitsARES Alexa Fluor Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.We also offer this two-step labeling technology in ourFISH Tag DNAandFISH Tag RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.

数据：

DNA microarray hybridized to DNA labeled using the ARES™ DNA Labeling Kits.DNA microarray hybridized to DNA labeled using the ARES™ DNA Labeling Kits. Total human RNA was labeled by reverse transcription using either ARES™ Alexa Fluor® 555 DNA Labeling Kit (A21677) or ARES™ Alexa Fluor® 647 DNA Labeling Kit (A21676). Labeled DNA was hybridized to a microarray containing human housekeeping genes. After hybridization, the array was imaged using a ScanArray 5000XL microarray scanner (PerkinElmer LAS, Inc.) using the appropriate lasers and filter sets. The image was pseudocolored so that white areas show the most intense signal and blue areas show the least intense signal.

Fluorescence in situ hybridization (FISH) mapping of a BAC clone on human metaphase chromosomes.Fluorescence in situ hybridization (FISH) mapping of a BAC clone on human metaphase chromosomes. FISH was performed using a BAC clone labeled using the ARES™ Alexa Fluor® 488 DNA Labeling Kit (A21665). The chromosomes were counterstained with DAPI (D1306, D3571, D21490). Image contributed by Nallasivam Palanisamy, Cancer Genetics Inc.

A cDNA microarray hybridized with three different cDNA samples labeled using our ARES™ DNA Labeling Kits.Three-color microarray. A cDNA microarray was hybridized with three different cDNA samples that were labeled using the ARES™ Alexa Fluor® 488 DNA Labeling Kit (Cat. no. A21665), the ARES™ Alexa Fluor® 594 DNA Labeling Kit (Cat. no. A21669) and the ARES™ Alexa Fluor® 647 DNA Labeling Kit (Cat. no. A21676), respectively. Each sample was imaged separately using a PerkinElmer ScanArray 5000 microarray scanner, and the black-and-white images were pseudocolored so that green represents the Alexa Fluor® 488 dye, red represents the Alexa Fluor® 594 dye and blue represents the Alexa Fluor® 647 dye. The images were overlaid using an additive RGB color scheme to reveal spots that show differential expression. Where fluorescence from all fluorophores is equivalent, spots are white; where blue is low, spots are yellow; where green is low, spots are pink; and where red is low, spots are aqua. Only a portion of the microarray is shown. Image provided by Gerti Schut and Mike Adams, University of Georgia.

参考文献：

1.Borrelia burgdorferi sigma54 is required for mammalian infection and vector transmission but not for tick colonization.2.Cold shock of a hyperthermophilic archaeon: Pyrococcus furiosus exhibits multiple responses to a suboptimal growth temperature with a key role for membrane-bound glycoproteins.3.Comparison of detection and signal amplification methods for DNA microarrays.

技术参数

产品优点 • Dye (Ex/Em): Alexa Fluor® 647 (650/670 nm) • Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides • Typically produces one dye per 12–20 bases • Optimal for FISH and microarrays

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Thermo Alexa Fluor 647 核酸标记,ARES Alexa Fluor 647 DNA Labeling Kit/Alexa Fluor 647 DNA标记试剂盒

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