SIRT1 Monoclonal Antibody (1F3)

货号：MA5-15677

规格：100 µL

价格：5682

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Purified recombinant fragment of human SIRT1 expre

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1F3

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1/200 - 1/400(Flow) ;1:200-1:1000(ICC);1/200 - 1/1000(IF);1/200 - 1/1000(IHC);1/500 - 1/2000(WB)

产品详细信息MA5-15677 targets SIRT1 in FACS, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.The MA5-15677 immunogen is purified recombinant fragment of human SIRT1 expressed in E. Coli.MA5-15677 detects SIRT1 which has a predicted molecular weight of approximately 120kDa.靶标信息NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).

数据：

SIRT1 Antibody (MA5-15677) in IFImmunofluorescence analysis of SIRT1 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SIRT1 Monoclonal Antibody (1F3) (Product # MA5-15677) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization of SIRT1. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. SIRT1 Antibody (MA5-15677) in IHCImmunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 monoclonal antibody (Product # MA5-15677) followed with DAB staining. SIRT1 Antibody (MA5-15677) in WBKnockdown of SIRT1 was achieved by transfecting MDA-MB-231 cells with SIRT1 specific siRNAs (Silencer® select Product # s223591, s223592). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the SIRT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SIRT1 Monoclonal Antibody (Product # MA5-15677, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SIRT1.

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