RAG2 Recombinant Rabbit Monoclonal Antibody (13H26L53)

货号：702837

规格：100 µg

价格：4643

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Protein corresponding to human RAG2 [aa271-aa527]

物种：人

宿主：兔

抗体亚型：IgG1, kappa

克隆号：13H26L53

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：
浓度：	0.5mg/mL	用法：	1:1000(WB）

产品详细信息This antibody is predicted to react with Mouse, Rat, Bovine.靶标信息RAG2 (V(D)J recombination-activating protein 2) is a core component of the RAG complex - a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity) and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: 1) first a nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends. 2) hairpin coding ends and 2 blunt, 5'-phosphorylated ends are created. The chromatin structure plays an essential rold in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and hair pinning expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin. This prevents accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. Mutations affecting the gene can results in combined cellular and humoral immune defects with granulomas, severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive, and Omenn syndrome.

数据：

RAG2 Antibody (702837) in WBWestern blot was performed using Anti-RAG2 Recombinant Rabbit Monoclonal Antibody (Product # 702837) and a ~62 kDa band corresponding to RAG2 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of JM1 (Lane 1), Jurkat (Lane 2), Raji (Lane 3), Ramos (Lane 4), KARPAS 299 (Lane 5), MOLT-4 (Lane 6), A549 (Lane 7) and HT-29 (Lane 8) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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