HP1 alpha Polyclonal Antibody

货号：PA5-88646

规格：100 µL

价格：4732

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant fusion protein containing a sequence c

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1.3mg/mL	用法：	1:50-1:200（IHC）；1:500-1:2000(WB）

产品详细信息Immunogen sequence: MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKETAK S; Positive Samples: HeLa, SH-SY5Y, A549, MCF-7; Cellular Location: Chromosome, Nucleus, centromere靶标信息Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

数据：

HP1 alpha Antibody (PA5-88646) in IHCImmunohistochemistry analysis of HP1 alpha in paraffin-embedded human brain astrocytoma using HP1 alpha Polyclonal Antibody (Product # PA5-88646) at a dilution of 1:100. HP1 alpha Antibody (PA5-88646) in WBWestern blot was performed using Anti-HP1 alpha Polyclonal Antibody (Product # PA5-88646) and a 25 kDa band corresponding to Chromobox protein homolog 5 was observed across tested cell lines with an uncharacterized band (*) at ~60 kDa. Nuclear enriched extracts (40 µg lysate) of MOLT-4 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), NIH/3T3 (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

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