| 产品详细信息Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma. |
| 1.Oncology lettersAccumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients."Published figure using CD33 monoclonal antibody (Product # 12-0339-42) in Flow Cytometry"2.CellGenetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia."Published figure using CD33 monoclonal antibody (Product # 12-0339-42) in Flow Cytometry"3.Nature communicationsKSRP specifies monocytic and granulocytic differentiation through regulating miR-129 biogenesis and RUNX1 expression."12-0339 was used in Flow cytometry/Cell sorting to elucidate how KH-Type Splicing Regulatory Protein, miR-129, and RUNX1 participate in a regulatory axis to control the outcome of myeloid differentiation."AuthorsZhao H,Wang X,Yi P,Si Y,Tan P,He J,Yu S,Ren Y,Ma Y,Zhang J,Wang D,Wang F,Yu J4.Blood cancer journalSwitching CAR T cells on and off: a novel modular platform for retargeting of T cells to AML blasts."12-0339 was used in Flow cytometry/Cell sorting to describe a novel modular universal platform for retargeting of T cells to AML blasts, via switching CD19-specific chimeric antigen receptor engineered T cells on or off."AuthorsCartellieri M,Feldmann A,Koristka S,Arndt C,Loff S,Ehninger A,von Bonin M,Bejestani EP,Ehninger G,Bachmann MP5.Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow TransplantationAntithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.AuthorsDabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J |
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