CD16/CD32 Monoclonal Antibody (93), CD16/CD32单克隆抗体/ FcR blocker FcR 阻断剂
货号：16-0161-81,16-0161-82,16-0161-85,16-0161-86,16-0161-38,16-0161-025,16-0161-050
规格：50μg,100μg,500μg,1mg,5mg,65mg,50mg
价格：934,1716,3434,5606,24089,72266,120443
产品类型：抗体和染料
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
荧光染料：其它
| 抗体类型 | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| 浓度： | 1 mg/mL | 用法： | 0.5 µg/test(Flow)；Assay-Dependent(FN)；Assay-Dependent (Neu) |
| 产品详细信息Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis. 93 has also been reported in blocking of Fc-mediated reactions in functional studies.Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found. |
| 数据 |
| CD16/CD32 Antibody (16-0161-85) in FlowStaining of BALB/c splenocytes with 0.25 µg Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg Anti-Mouse CD16/CD32 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Frontiers in immunologyContribution of IL-1RI Signaling to Protection againstCryptococcus neoformans52D in a Mouse Model of Infection."16-0161 was used in Flow cytometry/Cell sorting to determine the role of IL-1RI signalling in protection against cryptococcal infection."AuthorsShourian M,Ralph B,Angers I,Sheppard DC,Qureshi ST2.Frontiers in immunologyProtein Deiminase 4 and CR3 RegulateAspergillus fumigatusand β-Glucan-Induced Neutrophil Extracellular Trap Formation, but Hyphal Killing Is Dependent Only on CR3."16-0161 was used in Flow cytometry/Cell sorting to conclude that PAD4 together with CR3-mediated ROS production is required for NET formation in response to A. fumigatus."AuthorsClark HL,Abbondante S,Minns MS,Greenberg EN,Sun Y,Pearlman E3.Stem cell reportsAnalysis of Runx1 Using Induced Gene Ablation Reveals Its Essential Role in Pre-liver HSC Development and Limitations of an InVivo Approach."16-0161 was used in Flow cytometry/Cell sorting to study tamoxifen-inducible Runx1 inactivation in vivo."AuthorsSenserrich J,Batsivari A,Rybtsov S,Gordon-Keylock S,Souilhol C,Buchholz F,Hills D,Zhao S,Medvinsky A4.OncotargetMyeloid cell expressed proprotein convertase FURIN attenuates inflammation."16016182 was used in blocking or activating experiment to investigate the role of FURIN in LysM positive myeloid cells in vivo"AuthorsCordova ZM,Grönholm A,Kytölä V,Taverniti V,Hämäläinen S,Aittomäki S,Niininen W,Junttila I,Ylipää A,Nykter M,Pesu M5.The Journal of clinical investigationPML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia."16016182 was used in blocking or activating experiment to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9"AuthorsCole CB,Verdoni AM,Ketkar S,Leight ER,Russler-Germain DA,Lamprecht TL,Demeter RT,Magrini V,Ley TJ |
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