CD3e Monoclonal Antibody (eBio500A2 (500A2)), Biotin, eBioscience™

货号：13-0033-82,13-0033-85,13-0033-86

规格：100 µg,500 µg,1 mg

价格：1215,2534,4560

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：eBio500A2 (500A2)

荧光染料：Biotin

类型:	一抗	同型对照：	Syrian Hamster IgG Isotype Control, Biotin, eBioscience™
浓度：	0.5 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3e Antibody (13-0033-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 17-0191-82) and 0.125 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 13-4914-81) (left) or 0.125 µg of Anti-Mouse CD3e Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.The Journal of biological chemistryLoss of Core 1-derived O-Glycans Decreases Breast Cancer Development in Mice."Published figure using CD3e monoclonal antibody (Product # 13-0033-82) in Immunohistochemistry"2.Journal of immunology (Baltimore, Md. : 1950)EBI2 operates independently of but in cooperation with CXCR5 and CCR7 to direct B cell migration and organization in follicles and the germinal center."13-0033 was used in Immunohistochemistry-immunofluorescence to show EBI2-mediated chemotaxis provides a third dimension to B cell migration that balances and integrates with the inputs from CXCR5 and CCR7 to determine B cell positioning."AuthorsGatto D,Wood K,Brink R3.Journal of immunology (Baltimore, Md. : 1950)EBI2 operates independently of but in cooperation with CXCR5 and CCR7 to direct B cell migration and organization in follicles and the germinal center."13-0033 was used in Immunohistochemistry-immunofluorescence to show EBI2-mediated chemotaxis provides a third dimension to B cell migration that balances and integrates with the inputs from CXCR5 and CCR7 to determine B cell positioning."AuthorsGatto D,Wood K,Brink R4.The Journal of experimental medicineThymus-autonomous T cell development in the absence of progenitor import."13-0033 was used in Flow cytometry/Cell sorting to characterise the previously unrecognised capacity of the thymus to sustain T cell development during bone marrow progenitor deprivation."AuthorsMartins VC,Ruggiero E,Schlenner SM,Madan V,Schmidt M,Fink PJ,von Kalle C,Rodewald HR5.The Journal of experimental medicineNotch, Id2, and RORγt sequentially orchestrate the fetal development of lymphoid tissue inducer cells."13-0033 was used in Magnetic cell separation to investigate the development of lymphoid tissue inducer (LTi) cells from common lymphoid progenitors (CLPs) in the fetal liver, showing that sequential changes in expression of Notch, Id2, and RORγt orchestrate the development of LTi cells."AuthorsCherrier M,Sawa S,Eberl G

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