K0651 Thermo DNA标记,Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

K0651 Thermo DNA标记,Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒

2024-10-24

Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒

货号：K0651,K0652

规格：10 reactions,30 reactions

价格：882,2261

产品类型：核酸标记

品牌：Thermo Fisher

Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.Biotin-labeled DNA is detected with theBiotin Chromogenic Detection Kitor conventional biotin-avidin or biotin-streptavidin detection systems.PrincipleRandom decamers are annealed to a denatured template DNA molecule, and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand.

优点：

- Non-radioactivelabeling of DNA- Efficientpriming of labeling reactions with random decamers- High yieldswith Klenow Fragment, exo-: no degradation of a labeled probe during reaction

数据：

图1.Dot-blot hybridization with a biotin-labeled probeLambda DNA/HindIII was biotin-labeled with the Biotin DecaLabel DNA Labeling Kit and used as a hybridization probe in a dot-blot of the homologous DNA on SensiBlot Plus Nylon Membrane. The blot was developed with the Biotin Chromogenic Detection Kit.

组成成分：

▪Klenow Fragment, exo–, 5 u/μl▪Decanucleotide in 5X Reaction Buffer▪Biotin Labeling Mix（1 mM dGTP, 1 mM dATP, 1 mM dCTP, 0.65 mM dTTP, 0.35 mM Biotin-11-dUTP aqueous solution）▪Control Template （λ DNA/HindIII fragments,10 ng/µl）▪Biotin-labeled DNA （50 µl of biotin-labeled DNA (λ DNA/HindIII), 5 ng/µl）▪Water, nuclease-free

K0651 Thermo DNA标记,Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒

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