F532L,Phusion High-Fidelity PCR Master Mix with GC Buffer/含GC缓冲液的Phusion高保真PCR混合物-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F532L,Phusion High-Fidelity PCR Master Mix with GC Buffer/含GC缓冲液的Phusion高保真PCR混合物

2024-10-24

Phusion High-Fidelity PCR Master Mix with GC Buffer/含GC缓冲液的Phusion高保真PCR混合物

货号：F532L,F532S

规格：500 reactions,100 reactions

价格：5056,1267

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that ofTaq, and 6-fold lower than that ofPfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Two master mix formulations are available - with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

优点：

- High fidelity (25XTaq)- Fast PCR due to short extension times (15-30 s/kb)- Robust performance, minimal optimization needed

数据：

图1.Superior yields of long PCR products20kb fragment from λ DNA and 7.5 kb fragment from human genomic DNA was amplified with Phusion and proofreading DNA polymerase from other suppliers. Phusion and Phusion Green DNA Polymerases were the only enzymes capable of providing high amounts of desired PCR products. In contrast, other proofreading polymerases delivered zero or significantly lower yields.

图2.Phusion DNA Polymerase combines extreme fidelity with unparalleled speed and robustnessA random set of 16 clones from aThermus sp.genomic library was amplified from bacterial colonies. The results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate ofPfuDNA polymerase was only 56% and that ofTaqDNA polymerase 62% with significantly lower yields.

图3.Extreme fidelity for demanding PCRRelative fidelity values of different DNA polymerases. Phusion DNA Polymerases have extremely low error rates. The error rate, determined by a modified lacI-based method, is approximately 50-fold lower than that ofTaqDNA polymerase and 6-fold lower than that ofPfuDNA polymerase. Fidelity = 1/error rate.

图4.Less enzyme - superior yieldA 3.8kb fragment from human beta globin gene was amplified with three different DNA polymerases. Phusion DNA Polymerase was able to amplify the 3.8kb genomic fragment with a combined annealing and extension step of only 1minute, thus being significantly faster than the two other polymerases tested. A single unit of Phusion DNA Polymerase produced higher yields than 2.5 or 5 units of the Pfu DNA polymerases.

组成成分：

▪10 x 1.25 mL Phusion Master Mix with GC Buffer (2X) (provides 1.5 mM MgCl2in final reaction)▪ 2 x 500 μL 100% DMSOStore at -20°C.

F532L,Phusion High-Fidelity PCR Master Mix with GC Buffer/含GC缓冲液的Phusion高保真PCR混合物

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