F531L PCR Thermo ,Phusion High-Fidelity PCR Master Mix with HF Buffer/含HF缓冲液的高保真PCR混合物-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F531L PCR Thermo ,Phusion High-Fidelity PCR Master Mix with HF Buffer/含HF缓冲液的高保真PCR混合物

2024-10-24

Phusion High-Fidelity PCR Master Mix with HF Buffer/含HF缓冲液的高保真PCR混合物

货号：F531L,F531S

规格：500 reactions,100 reactions

价格：5055,1267

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion 高保真 DNA 聚合酶为高效 PCR 设立了“金标准”。Phusion 高保真 DNA 聚合酶的错误率是Taq的 1/50，是Pfu的 1/6，因此是克隆和其他需要高保真度的应用的极佳之选。与其他 DNA 聚合酶相比，Phusion DNA 聚合酶性能稳定，实验方案耗时少（即使存在 PCR 抑制剂），能以更低的酶量实现更高的产出。Phusion 高保真 PCR 预混液是方便操作的 2X 混合液，其含有 Phusion DNA 聚合酶、核苷酸以及含 MgCl2的优化反应缓冲液。提供两种预混液配方 - 含 HF 缓冲液（F-531S 和 F-531L）和含 GC 缓冲液（F-532S 和 F-532L）。HF 缓冲液中的 Phusion DNA 聚合酶的错配率 (4.4 × 10-7) 低于 GC 缓冲液的错配率 (9.5 × 10-7)。因此，应该使用含 HF 缓冲液的预混液作为高保真扩增时的默认预混液。但是，GC 缓冲液可以提高 Phusion DNA 聚合酶对一些复杂模板或长模板的扩增性能，例如富含 GC 的模板或具有复杂二级结构的模板。应用•高保真 PCR•扩增复杂（高 GC 含量）模板• 克隆•生成测序模板•长片段 PCR（长达 20 kb）•突变•高通量 PCR

优点：

- 高保真度 (50XTaq)- 延伸时间短 (15-30 s/kb)，可实现快速 PCR- 性能稳定，几乎无需优化

数据：

图1.Superior yields of long PCR products20kb fragment from λ DNA and 7.5 kb fragment from human genomic DNA was amplified with Phusion and proofreading DNA polymerase from other suppliers. Phusion and Phusion Green DNA Polymerases were the only enzymes capable of providing high amounts of desired PCR products. In contrast, other proofreading polymerases delivered zero or significantly lower yields.

图2.Phusion DNA Polymerase combines extreme fidelity with unparalleled speed and robustnessA random set of 16 clones from aThermus sp.genomic library was amplified from bacterial colonies. The results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate ofPfuDNA polymerase was only 56% and that ofTaqDNA polymerase 62% with significantly lower yields.

图3.Extreme fidelity for demanding PCRRelative fidelity values of different DNA polymerases. Phusion DNA Polymerases have extremely low error rates. The error rate, determined by a modified lacI-based method, is approximately 50-fold lower than that ofTaqDNA polymerase and 6-fold lower than that ofPfuDNA polymerase. Fidelity = 1/error rate.

图4.Less enzyme - superior yieldA 3.8kb fragment from human beta globin gene was amplified with three different DNA polymerases. Phusion DNA Polymerase was able to amplify the 3.8kb genomic fragment with a combined annealing and extension step of only 1minute, thus being significantly faster than the two other polymerases tested. A single unit of Phusion DNA Polymerase produced higher yields than 2.5 or 5 units of the Pfu DNA polymerases.

组成成分：

- 10 x 1.25 mL Phusion 预混液（含 HF 缓冲液） (2X)（在最终反应中提供 1.5 mM MgCl₂）- 2 x 500 μL 100% DMSO储存于 -20°C。

F531L PCR Thermo ,Phusion High-Fidelity PCR Master Mix with HF Buffer/含HF缓冲液的高保真PCR混合物

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