2. 试剂
  3. F520L Thermo HF缓冲液,Phusion HF Buffer Pack, detergent-free/Phusion HF缓冲液包,无洗涤剂

F520L Thermo HF缓冲液,Phusion HF Buffer Pack, detergent-free/Phusion HF缓冲液包,无洗涤剂

2024-10-24
Phusion HF Buffer Pack, detergent-free/Phusion HF缓冲液包,无洗涤剂

货号：F520L

规格：4 x 1.5 mL

价格：333

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

组成成分：

▪Detergent-free 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl2)▪ 50 mM MgCl2▪ DMSOStore at -20°C.

相关产品：

▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化

技术参数
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