CD360 (IL-21 Receptor) Monoclonal Antibody (2SX21R), PerCP-eFluor 710, eBioscience

货号：46-3601-42,46-3601-41

规格：100Tests,25 Tests

市场价格：3402,1442

产品类型：流式抗体

品牌：eBioscience

抗原：CD360

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2SX21R

荧光染料：PerCP-eFluor™ 710

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: This 2SX21R monoclonal antibody reacts with human CD360 (Interleukin-21 Receptor, IL-21R). IL-21R forms a heterodimeric receptor complex with the common gamma chain to form a functional receptor for IL-21. IL-21 is a class I cytokine of the common gamma chain family of cytokines and signals through the IL-21R complex to activate the JAK/STAT (Janus Kinase/Signal Transducers and Activators of Transcription) signaling pathway. Activation of the JAK/STAT pathway by IL-21 promotes transcription of genes responsive to STAT1 and STAT3. IL-21 is mainly produced by CD4+ T cells and mediates the proliferation, differentiation, and effector functions of cells expressing IL-21R such as B cells, T cells, NK cells, dendritic cells and other populations of myeloid cells. IL-21 and its receptor are implicated in the pathogenesis of chronic viral infections, autoimmunity, allergy, organ transplantation, and cancer.Applications Reported: This 2SX21R antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2SX21R antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2 (IL2) and interleukin 5 (IL5). This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants encoding the same protein have been described.仅用于科研。不用于诊断过程。

数据

CD360 (IL-21 Receptor) Antibody (46-3601-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0198-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD360 (IL-21 Receptor) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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技术参数

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