产品介绍 类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7浓度: 5 µL/Test用法:5 µL (0.5 µg)/test(Flow)产品详细信息Description: The eBioSN3 (SN3 A5-2H10) monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HAS). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.Applications Reported: This eBioSN3 (SN3 A5-2H10) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, andcontrol of autoimmunity.数据
CD24 Antibody (25-0247-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD24 PE-Cyanine7 (purple histogram). Cells in the granulocyte gate were used for analysis.推荐产品二抗荧光二抗免疫组化一抗标签抗体参考文献1. OncogeneTFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.2. TheranosticsGlutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.3. BMC biologyThe Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.4. Breast cancer research : BCRThe hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells.5. Molecular cancerHOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.
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