18064-014INVITROGEN反转录酶II,SuperScript II Reverse Transcript

2024-07-28

金山科研平台授权一级代理,询价咨询微信jinshanbio
INVITROGEN反转录酶II,SuperScript II Reverse Transcriptase货号18061014规格:10,000 units现货供应

金山科研平台,咨询微信:jinshanbio询价,现货促销

INVITROGEN反转录酶II,SuperScript II Reverse Transcriptase货号18061014

描述反转录(RT)superscript®II是一个改进版本superscript®RT。It is a聚合酶,DNA链synthesizes有互补单链RNA从DNA、RNA、DNA杂交。这种酶是转基因工程by the introduction of点突变in the RNase H活性中心to reduce RNase H活性。这eliminates降解RNA分子结构的变化在*链cDNA Synthesis and gives superscript®II RT性能特性上。

INVITROGEN反转录酶II,SuperScript II Reverse Transcriptase货号18061014

描述

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.•Yield– Reduced RNase H activity results in greater first-strand cDNA yields•Size of cDNA– Generation of RT-PCR products up to 12 kb•Thermostability– Full activity at 42°C•Flexibility– Uses either total or poly(A)+RNAPerformance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18as template•primer (3).Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

ase货号18061014

订购信息:

货号

单位
18064014也被称为 18064-014

10,000 units

18064022也被称为 18064-022

2,000 units

18064071也被称为 18064-071

4 x 10,000 units

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-29967.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价