ScienCellGK096人类雪旺细胞生物学qPCR检测试剂盒的特点

2024-08-08

GK096 ScienCell 人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK096 ScienCell人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK096

产品名称:GK096 ScienCell人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

北京裕恒丰科技有限公司是ScienCell中国区总代理,提供各种基因表达分析相关品,ScienCell细胞培养基、转染试剂、细胞检测试剂盒等细胞生物学产品。

GK096 ScienCell人类雪旺细胞生物学qPCR检测试剂盒,Human Schwann Cell Biology qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ Human Schwann Cell Biology qPCR Array Kit (GQH-SCH) 旨在促进参与人类雪旺细胞生物学的 88 个关键基因的基因表达谱分析,例如周围神经形成和再生、雪旺细胞发育和分化、髓鞘形成和细胞外基质 (ECM) 合成。与选定的雪旺细胞相关疾病有关的基因也包含在阵列中。下面显示了如何根据其功能对基因进行分组的简要示例:

雪旺细胞标记物: SOX10, NGFR, SOX2, GAP43, NCAM1, EGR2, POU3F1, MBP, MPZ, S100B, PMP22, GFAP

雪旺细胞髓鞘形成:MOG,CNP,SOX10,EGR2,NFATC4,NRG1,CD9,HEXB,IFNG,NTF3,PLP1

成熟和分化:WASL,NCAM1,VGF,IL6,BDNF,STAT1,PTN,CD40,LIF,MEF2C

雪旺细胞可塑性和去分化:ERBB2,RAF1,MAPK1,MAPK3,MAPK11,MAPK14,JUN,ARTN,BDNF,GDNF,SOX2,GJA1

神经再生支持: NTN1, UNC5B, SHH, GAP43, CDH1, TYRP1, FABP7, SOSTDC1, KCNJ10, LRRTM4, OLIG1

周围神经干中的ECM合成:层粘连蛋白,胶原蛋白,FN1,ICAM1,PLAT,PLAUR,NRG1,ITGB1,ERBB2,ERBB3

与以下因素有关的基因

遗传性运动和感觉:WNK1,KIF1A,FAM134B,SCN9A,APOB,IKBKAP,IFNB1,HLA,AQP4,PLP1,IFNG

神经鞘瘤: NF1, NF2, SMARCB1, KIT, PRKAR1A, GFAP, EZR, SOX10, NGFR, MBP

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Schwann Cell Biology qPCR Array Kit (GQH-SCH) is designed to facilitate gene expression profiling of 88 key genes involved in human Schwann cell biology, such as peripheral nerve formation and regeneration, Schwann cell development and differentiation, myelination and extracellular matrix (ECM) synthesis. Genes implicated in selected Schwann cell related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:

· Schwann cell markers: SOX10, NGFR, SOX2, GAP43, NCAM1, EGR2, POU3F1, MBP, MPZ, S100B, PMP22, GFAP

· Schwann cell myelination: MOG, CNP, SOX10, EGR2, NFATC4, NRG1, CD9, HEXB, IFNG, NTF3, PLP1

· Maturation and differentiation: WASL, NCAM1, VGF, IL6, BDNF, STAT1, PTN, CD40, LIF, MEF2C

· Schwann cell plasticity and dedifferentiation: ERBB2, RAF1, MAPK1, MAPK3, MAPK11, MAPK14, JUN, ARTN, BDNF, GDNF, SOX2, GJA1

· Nerve regeneration support: NTN1, UNC5B, SHH, GAP43, CDH1, TYRP1, FABP7, SOSTDC1, KCNJ10, LRRTM4, OLIG1

· ECM synthesis in the peripheral nerve trunk: laminins, collagens, FN1, ICAM1, PLAT, PLAUR, NRG1, ITGB1, ERBB2, ERBB3

· Genes implicated in

o hereditary motor and sensory neuropathies: WNK1, KIF1A, FAM134B, SCN9A, APOB, IKBKAP, IFNB1, HLAs, AQP4, PLP1, IFNG

o schwannoma: NF1, NF2, SMARCB1, KIT, PRKAR1A, GFAP, EZR, SOX10, NGFR, MBP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人雪旺细胞生物学 qPCR 阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

APOB

CNP

ERBB3

GJA1

IFNG

LAMA1

MAPK11

NCAM1

OLIG1

RAF1

SOX2

β-actin

B

AQP4

COL1A1

EZR

GZMB

IGFBP2

LAMA2

MAPK14

NF1

PLAT

S100B

STAT1

GAPDH

C

ARTN

COL1A2

FABP7

HEXB

IKBKAP

LAMB1

MAPK3

NF2

PLAUR

SCN9A

TGFB1

LDHA

D

BDNF

COL3A1

FAM134B

HLA-DQA1

IL6

LAMB2

MBP

NFATC4

PLP1

SHH

TYRP1

NONO

E

CD40

COL4A1

FN1

HLA-DQB1

ITGB1

LIF

MEF2C

NGFR

PMP22

SMARCB1

UNC5B

PPIH

F

CD9

COL4A2

GAP43

HLA-DRB1

JUN

LRRTM4

MOG

NRG1

POU3F1

SOD1

VGF

GDC

G

CDH1

EGR2

GDNF

ICAM1

KCNJ10

MAL

MPZ

NTF3

PRKAR1A

SOSTDC1

WASL

PPC

H

CHST8

ERBB2

GFAP

IFNB1

KIT

MAPK1

MYRF

NTN1

PTN

SOX10

WNK1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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GK029 Human Tie2/Angpt信号qPCR检测试剂盒,Human Tie2/Angpt Signaling qPCR Array Kit

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GK093 Human Renal Mesangial Cell Biology qPCR Array Kit,Human Renal Mesangial Cell Biology qPCR Array Kit

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