ScienCellGK087人类染色质组织和重塑qPCR检测试剂盒

2024-08-08

GK087 ScienCell 人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK087 ScienCell人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK087

产品名称:GK087 ScienCell人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK087 ScienCell人类染色质组织和重塑qPCR检测试剂盒,Human Chromatin Organization and Remodeling qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人类染色质组织和重塑qPCR阵列试剂盒(GQH-CHM)分析了88个参与调节染色质结构的关键基因。 染色质压缩DNA,允许有丝分裂,同时防止DNA损伤和控制基因表达。 它是一种复杂的大分子,仅在由RNA,DNA和蛋白质组成的真核生物中发现。 染色质的基本蛋白质是组蛋白,组蛋白修饰有助于改变染色质堆积和结构。 如何根据其功能对所包含的基因进行分组的简要示例如下所示:

染色质重塑:ATRX,CBX2,CREBBP,EP300,MECOM

染色质组织:HIST1H3A,HIST1H3J,HIST2H3A,HIST2H3D,HIST3H3

组蛋白乙酰化/去乙酰化:ELP3、HDAC1、HDAC4、JADE2、KAT6A

组蛋白甲基化/去甲基化:DNMT3A、DOT1L、EZH2、JMJD6、KDM6A

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Chromatin Organization and Remodeling qPCR Array Kit (GQH-CHM) profiles 88 key genes involved in regulating chromatin structure. Chromatin compacts DNA, allowing mitosis while preventing DNA damage and controlling gene expression. It is a complex macromolecule that is only found in eukaryotes consisting of RNA, DNA, and protein. The basic proteins of chromatin are histones and histone modifications contribute to altering chromatin packing and structure. Brief examples of how included genes may be grouped according to their function are shown below:

· Chromatin remodeling: ATRX, CBX2, CREBBP, EP300, MECOM

· Chromatin organization: HIST1H3A, HIST1H3J, HIST2H3A, HIST2H3D, HIST3H3

· Histone acetylation/deacetylation: ELP3, HDAC1, HDAC4, JADE2, KAT6A

· Histone methylation/demethylation: DNMT3A, DOT1L, EZH2, JMJD6, KDM6A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人类染色质组织和重塑 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ACKR1

CCL4

CSF2

EPO

GYPB

ICAM1

IL17A

IL6

NLRP3

SLC4A1

TLR4

β-actin

B

ALB

CCL5

CSF3

F3

GYPC

IFNAR1

IL18

IRF1

NOS2

TFRC

TLR9

GAPDH

C

ANGPT1

CD36

CTLA4

FASLG

HAVCR2

IFNG

IL1A

ITGAL

ORC1

TGFB1

TNF

LDHA

D

ANGPT2

CD40LG

CXCL10

FCGR2A

HBA2

IFNGR1

IL1B

LTA

PECAM1

TGFB2

TNFRSF1A

NONO

E

C5AR1

CD55

CXCL8

FCGR2B

HLA-B

IL10

IL1RN

MIF

PF4

THBD

TNFRSF1B

PPIH

F

CCL11

CD69

CXCL9

FOXP3

HMOX1

IL12A

IL2

MMP9

PROCR

THBS1

VCAM1

GDC

G

CCL2

CD81

CXCR3

G6PD

HP

IL12B

IL4

MYD88

SELE

TIRAP

VEGFA

PPC

H

CCL3

CR1

DHFR

GYPA

HRH1

IL13

IL5

NCR3

SELP

TLR2

VWF

NTC

提示:基因选择可能会根据新的研究和开发进行更新

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