ScienCellGK063人细胞周期蛋白依赖性激酶检测试剂盒

2024-08-08

GK063 ScienCell 人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK063 ScienCell人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK063

产品名称:GK063 ScienCell人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK063 ScienCell人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒,Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit, 产品说明:

ScienCell 的 GeneQuery™ 人细胞周期蛋白和细胞周期蛋白依赖性激酶 qPCR 阵列试剂盒 (GQH-CCN) 旨在促进 40 种选定细胞周期蛋白和细胞周期蛋白依赖性激酶 (CDK) 的基因表达谱分析。CDK是细胞周期进程和细胞分裂所需的一类小酶,通过其底物的磷酸化。CDK必须与细胞周期蛋白结合才能激活。细胞周期蛋白/CDK复合物调节细胞周期检查点,是细胞周期进程的驱动力。下面显示了如何对所包含的基因进行分组的简要示例:

细胞周期蛋白: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY

CDK: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可以特异性识别并有效扩增靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+,不含DMSO);(ii)除非另有说明,否则引物组可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit (GQH-CCN) is designed to facilitate gene expression profiling of 40 selected cyclins and cyclin-dependent kinases (CDKs). CDKs are a family of small enzymes required for cell cycle progression and cell division, by means of phosphorylation of their substrates. CDKs must bind to cyclins to become fully activated. Cyclin/CDK complexes regulate cell cycle checkpoints and are the driving force of cell cycle progression. Brief examples of how included genes may be grouped are shown below:

· Cyclins: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY

· CDKs: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查询™人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR阵列板布局*(8 个粗体和斜体控件)

1

2

3

4

5

6

7

8

9

10

11

12

A

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

B

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

C

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

D

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

E

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

F

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

G

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

H

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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