AnaSpec AS-72113 AnaTag™ R - PE 现货

2024-08-08

AnaSpec AS-72113 AnaTag™ R - PE Labeling Kit - 1 kit,AnaTag™ R-PE标签试剂盒-1试剂盒

AnaSpec ( 美国 )

AnaSpec公司成立于1993年,是一家为全球生命科学研究,提供综合蛋白质组学和基因组学解决方案**供应商。 公司产品主要分三大部分:提供专业多肽,检测试剂,抗体检测试剂盒;适合基础研究的高通量筛选和新药物研发相关试剂;多肽合成,抗体生产,寡核苷酸的合成等定制服务。

我们作为AnaSpec公司的代理商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

AnaSpec AS-72113 AnaTag™ R - PE Labeling Kit - 1 kit,AnaTag™ R-PE标签试剂盒-1试剂盒,产品简介:

产品品牌:AnaSpec

产品名称:AnaTag™ R-PE标签试剂盒-1试剂盒

英文名称:AnaTag™ R - PE Labeling Kit - 1 kit

产品货号:AS-72113

产品规格:1 kit

AnaSpec AS-72113 AnaTag™ R - PE Labeling Kit - 1 kit,AnaTag™ R-PE标签试剂盒-1试剂盒,产品说明:

The AnaTag™ R-PE Labeling Kit is optimized for use in the conjugation of R-Phycoerythrin (R-PE) to antibodies. R-PE, a fluorescent protein from phycobiliprotein family, has broad absorption bands with peaks at 565 nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1), and 539 nm (eM =1.62 X 106 M-1cm-1); it therefore can be excited with versatile excitation sources. Its maximal emission can be detected at 578 nm. The broad excitation spectrum also provides the advantage of multi-color immunofluorescent staining or cell sorting. R-PE and the closely related B-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. The AnaTagTM R-PE Labeling Kit contains SH-reactive R-PE. SMCC modified R-PE reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. The amount of R-PE supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Native and SMCC-activated R-PE are also available as separate products.

Note: AnaTag kits are not intended for peptide labeling.

The AnaTag™ R-PE标记试剂盒经过优化,可用于R-藻红蛋白(R-PE)与抗体的结合。R-PE是藻胆蛋白家族的一种荧光蛋白,具有宽的吸收带,峰值为565nm(eM=1.96x106m-1cm-1)、498(eM=1.53x106m-1cm-1)和539nm(eM=1.62x106m-1cm-1);因此,可以使用多种激励源对其进行激励。其最大发射波长为578nm。宽激发光谱还提供了多色免疫荧光染色或细胞分选的优势。R-PE和密切相关的B-PE是荧光*的藻胆蛋白,具有橙色荧光。它们比传统的有机荧光团更明亮、更耐光。AnaTagTM R-PE标记试剂盒包含SH反应性R-PE。SMCC修饰的R-PE与目标抗体的巯基反应,无需额外活化,从而简化接合方案。该试剂盒中提供的R-PE量足以标记高达1mg的抗体。该试剂盒提供所有试剂、纯化柱和详细的分步方案。天然和SMCC活化R-PE也可作为单独的产品提供。

注:AnaTag试剂盒不用于肽标记。

成分A:活化R-PE(SMCC活化交联R-藻红蛋白):3 mg,10 mg/mL组分B:DTT:0.1 mL组分C:旋转柱:1柱组分D:洗涤管:1管组分E:收集管:1管组分F:重力柱:1柱组分G:DMSO:1 mL组分H:NEM以阻断游离巯基:1小瓶组分I:用于B-PE与蛋白质接合的反应缓冲液:100 mL

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