AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*,SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*,1 kit
AnaSpec ( 美国 )
AnaSpec公司成立于1993年,是一家为全球生命科学研究,提供综合蛋白质组学和基因组学解决方案的lingxian供应商。 公司产品主要分三大部分:提供专业多肽,检测试剂,抗体检测试剂盒;适合基础研究的高通量筛选和新药物研发相关试剂;多肽合成,抗体生产,寡核苷酸的合成等定制服务。
我们作为AnaSpec公司的代理商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*,SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*,1 kit,产品简介:
产品品牌:AnaSpec
产品名称:SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*
英文名称:SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*
产品货号:AS-72263
产品规格:1 kit
AnaSpec AS-72263 SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit *Fluorimetric*,SensoLyte®SARS-CoV-2类木瓜蛋白酶(PLpro)活性测定试剂盒*荧光法*,1 kit,产品说明:
SensoLyte®520 SARS-CoV-2类木瓜蛋白酶/去泛素酶活性检测试剂盒采用荧光肽底物检测去泛素化活性。当活性木瓜蛋白酶样蛋白酶切割底物时,在激发/发射=490 nm/520 nm时,会导致亮绿色荧光增加。该检测方法可检测样品中低至7.8 ng/mL的活性木瓜蛋白酶。该试剂盒可用于检测纯化酶制剂、生物样品中的酶活性,也可用于化合物筛选。
SARS-Cov-2木瓜蛋白酶样蛋白酶(PLpro)编码为非结构蛋白3(nsp3),参与病毒多蛋白的切割。这种酶水解泛素链,泛素链在炎症过程中起重要作用,并从蛋白质中去除干扰素刺激的基因15(ISG15)修饰以逆转抗病毒反应。PLpro作为SARS-CoV-2复制和感染宿主过程中的一种必需酶,已成为冠状病毒抑制剂开发的一个有吸引力的药物靶点。
The SensoLyte® 520 SARS-CoV-2 Papain-like Protease/ Deubiquitinase Activity Assay Kit employs a fluorescent peptide substrate for the detection of deubiquitnation activity. When active Papain-like Protease cleaves the substrate, it results in an increase of bright green fluorescence at excitation/emission = 490 nm/520 nm. This assay can detect as low as 7.8 ng/mL active Papain-like Protease in the sample. This kit can be used to detect enzyme activity in purified enzyme preparations, biological samples and can also be applied for the compound screening.
SARS-Cov-2 Papain-like Protease (PLpro) is encoded as non-structural protein 3 (nsp3) and it is involved in the cleavage of the viral polyprotein. This enzyme hydrolyzes ubiquitin chains which are important in the inflammation process and also removes interferon-stimulated gene 15 (ISG15) modifications from proteins to reverse antiviral responses. As an essential enzyme in the process of SARS-CoV-2 replication and infection of the host, PLpro has been an attractive drug target for the coronavirus inhibitor development.
Kits components 产品组件:
Component A: Papain-like Protease Deubiquitination substrate Ex/Em=490 nm/520 nm upon cleavage; 400µM, 50µL Component B: Rh110 fluorescence reference standard, Ex/Em=490 nm/520 nm; 400µM, 15µL Component C: Recombinant SARS-CoV-2 Papain-like protease; 0.1mg/mL, 10µL Component D: 2X Assay Buffer, 25mL Component E: Inhibitor (GRL0617); 10µM, 10µL
组分A:木瓜蛋白酶类去泛素化底物Ex/Em=490nm/520nm;400µM,50µL组分B:Rh110荧光对照品,Ex/Em=490 nm/520 nm;400µM,15µL组分C:重组SARS-CoV-2木瓜蛋白酶;0.1mg/mL,10µL组分D:2X分析缓冲液,25mL组分E:抑制剂(GRL0617);10µM,10µL
AnaSpec品牌部分产品货号:
品牌 | 货号 | 名称 | 规格 |
AnaSpec | AS-72262 | SensoLyte® SARS-CoV-2 3CL Protease Activity Assay Kit *Fluorimetric*, | 1 kit |
AnaSpec | AS-72263 | SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit | 1 kit |
AnaSpec | AS-55551 | SensoLyte® Anti-Human β-Amyloid (1-40) Quantitative ELISA Kit *Colorimetric* | 1 kit |
AnaSpec | AS-55552 | SensoLyte® Anti-Human β-Amyloid (1-42) Quantitative ELISA Kit *Colorimetric* | 1 kit |
AnaSpec | AS-72246 | AnaTag™ Europium Protein Labeling Kit | 1 kit |
AnaSpec | AS-72247 | AnaTag™ Protein Labeling Kit for TR-FRET - 1 kit | 1 kit |
AnaSpec | AS-71120 | SensoLyte® AMC Caspase Profiling Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71121 | SensoLyte® AMC Caspase Substrate Sampler Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71124 | SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71126 | SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays - 1 kit | 1 kit |
AnaSpec | AS-71127 | SensoLyte® 490 HIV Protease Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71128 | SensoLyte® 490 MMP-1 Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-71129 | SensoLyte® 490 MMP-2 Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-72040 | SensoLyte® 520 Renin Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-72122 | SensoLyte® Luminescent Alkaline Phosphatase Assay Kit Luminometric - 1 kit | 1 kit |
AnaSpec | AS-72129 | SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric - 1 kit | 1 kit |
AnaSpec | AS-20276 | Beta-Amyloid (1-42) | 1 mg |
AnaSpec | AS-20276-25 | Beta-Amyloid (1-42) | 25 mg |
AnaSpec | AS-20276-5 | Beta-Amyloid (1-42) | 5 mg |
AnaSpec | AS-20291 | Threonine Phosphopeptide, PKC Substrate 4, phosphorylated - 1 mg | 1 mg |
AnaSpec | AS-20292 | Insulin Receptor (1142-1153), pTyr1146 - 1 mg | 1 mg |
AnaSpec | AS-21158 | HA peptide | 5 mg |
AnaSpec | AS-24467 | Exendin (9-39) | 0.5 mg |
AnaSpec | AS-24468 | Exendin (9-39) | 1 mg |
AnaSpec | AS-64129-05 | Beta-Amyloid (1-42) • HFIP | 0.5 mg |
AnaSpec | AS-64129-1 | Beta-Amyloid (1-42) • HFIP | 1 mg |
AnaSpec | AS-72215 | AggreSure ß-Amyloid (1-40), human - 0.25 mg | 0.25 mg NET peptide |
AnaSpec | AS-72216 | AggreSure ß-Amyloid (1-42), human - 0.25 mg | 0.25 mg NET peptide |
AnaSpec | AS-72049 | AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-72050 | AnaTag™ HiLyte™ Fluor 647 Microscale Protein Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-72111 | AnaTag™ APC Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-72113 | AnaTag™ R - PE Labeling Kit - 1 kit | 1 kit |
AnaSpec | AS-55158-100 | Recombinant human MOG (1-125) | 100 µg |
AnaSpec | AS-55158-1000 | Recombinant human MOG (1-125) | 1000 µg |
AnaSpec | AS-55158-500 | Recombinant human MOG (1-125) | 500 µg |
AnaSpec | AS-55525-1 | MMP-12 (Catalytic Domain), human recombinant | 1 µg |
AnaSpec | AS-55525-10 | MMP-12 (Catalytic Domain), human recombinant | 10 µg |
AnaSpec | AS-55525-50 | MMP-12 (Catalytic Domain), human recombinant | 50 µg |
AnaSpec | AS-72237 | Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg | 1 mg |
AnaSpec | AS-72239 | Protein A-HiLyte™ Fluor 750 Conjugate - 1 mg | 1 mg |
AnaSpec | AS-72237 | Protein A-HiLyte™ Fluor 647 Conjugate - 1 mg | 1 mg |
AnaSpec | AS-72239 | Protein A-HiLyte™ Fluor 750 Conjugate - 1 mg | 1 mg |
如需其他产品请联系微信:jinshanbio。
©
bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://gibcohyclone.cn/thread-36580.htm