Rat anti Human CD28 antibody, clone YTH913.12

货号：MCA709SBV515

规格：100 Tests/0.5ml

价格：3808

产品类型：流式抗体

品牌：Bio-Rad

物种：人

宿主：大鼠

抗体亚型：IgG2b

克隆号：YTH913.12

荧光染料：StarBright Violet 515

类型:	单抗	同型对照：	IgG2b
浓度：		用法：	5 µL/test(Flow)
免疫原性：	Human peripheral blood T-cells

Rat anti Human CD28 antibody, clone YTH913.12recognizes human CD28, a ~44 kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompsonet al.1989), responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Violaet al.1996).CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azumaet al.1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummelet al.1995).Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauriet al1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived NK cells using cloneYTH913.12 (Wilson et al. 1999).

数据

Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A488) and SBV515 conjugated Rat anti Human CD28 (MCA709SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on theZE5 Cell Analyzer.

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参考文献：

1. Reiter, C. (1989) Cluster Report: CD28 in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr.Oxford University Press. pp 352-3.2. Galea-Lauri, J.et al.(1999) Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells.J Immunol. 163 (1): 62-70.3. Gabdoulkhakova, A.et al.(2007) High rate of mutation reporter gene inactivation during human T cell proliferation.Immunogenetics. 59: 135-43.4.Pidgeon, C.et al.(2011) Regulation of IL-17 in chronic inflammation in the human lung.Clin Sci (Lond). 120: 515-24.5. Blanco, B.et al.(2003) Induction of human T lymphocyte cytotoxicity and inhibition of tumor growth by tumor-specific diabody-based molecules secreted from gene-modified bystander cells.J Immunol. 171: 1070-7.6. Costa, C.et al.(2002) Human NK cell-mediated cytotoxicity triggered by CD86 and Gal alpha 1,3-Gal is inhibited in genetically modified porcine cells.J Immunol. 168: 3808-16.7. Litjens, N.H.et al.(2011) Identification of Circulating Human Antigen-Reactive CD4+FOXP3+ Natural Regulatory T Cells.J Immunol. 188: 1083-90.8. Goodier, M.R. and Londei, M. (2004) CD28 is not directly involved in the response of human CD3- CD56+ natural killer cells to lipopolysaccharide: a role for T cells.Immunology. 111: 384-90.9. Wilson, J.L.et al.(1999) NK cell triggering by the human costimulatory molecules CD80 and CD86.J Immunol. 163: 4207-12.10. Kropf, P.et al.(2007) Arginase activity mediates reversible T cell hyporesponsiveness in human pregnancy.Eur J Immunol. 37: 935-45.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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