Bio-Rad mouse anti Rat CD45 MCA43,mouse anti Rat CD45

2024-10-22

mouse anti Rat CD45

货号:MCA43R,MCA43GA,MCA43G

规格:0.25 mg,0.1 mg,1 mg

价格:3018,2015,5398

产品类型:流式抗体

品牌:Bio-Rad

抗原: CD45

物种:大鼠

宿主:小鼠

抗体亚型:IgG1

克隆号:OX-1

荧光染料:Purified

产品介绍
Mouse anti Rat CD45 antibody, clone OX-1recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.This product is routinely tested in flow cytometry on rat splenocytes
产品详情
Target SpeciesRat
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogenRat thymocyte membrane glycoproteins.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Dick, A.D.et al.(2001) Distribution of OX2 antigen and OX2 receptor within retina.Invest Ophthalmol Vis Sci. 42: 170-6.2.Kurozumi, K.et al.(2007) Effect of tumor microenvironment modulation on the efficacy of oncolytic virus therapy.J Natl Cancer Inst. 99: 1768-81.3.Sunderland, C.A.et al.(1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.Eur J Immunol. 9 (2): 155-9.4.Woollett, G.R.et al.(1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.Eur J Immunol. 15 (2): 168-73.

技术参数

产品应用 IHC(F);IF;Flow

产品类型 Monoclonal Antibody

推荐用于 IHC(F);IF;Flow

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