BIO-RAD MCA275A700 Mouse anti Rat CD11b, clone OX-42,Mouse anti Rat CD11b, clone OX-42

2024-10-22

Mouse anti Rat CD11b, clone OX-42

货号:MCA275A700

规格:100 Tests/1ml

价格:3553

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD11b

物种:大鼠

宿主:小鼠

抗体亚型:IgG2a

克隆号:OX-42

荧光染料:Alexa Fluor 700

产品介绍
Mouse anti Rat CD11b, clone OX-42recognizes rat CD11b, also known asintegrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a singlevWFAdomain and multipleFG-GAPrepeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes andmicrogliain the brain (Robinsonet al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinsonet al.1986) as well as inhibit myelin binding and uptake (van der Laanet al.1996).
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to Alexa Fluor 700 - liquid
PreparationPurified IgG prepared by affinity chromatography on protein G from tissue culture supernatant.
Buffer SolutionPhosphate buffered saline
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Preservative Stabilisers0.09% Sodium Azide (NaN3)1%Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Alexa Fluor®700702723
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
DescriptionProduct CodeApplicationsPack Size
Mouse IgG2a Negative Control:Alexa Fluor® 700MCA1210A700F100 Tests/1ml
文献
1.Helming, L.et al.(2009) The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion.J Cell Sci. 122 (Pt 4): 453-9.2.Mwaikambo, B.R.et al.(2009) Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms.J Biol Chem. 284: 26695-707.3.Yang, C.N.et al.(2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.Neurobiol Dis. 42 (3): 221-30.4.Seeds, R.E.et al.(2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.Int Immunopharmacol. 11: 794-801.5.Samovski, D.et al.(2012) Insulin and AMPK regulate FA translocase/CD36 plasma membrane recruitment in cardiomyocytes via Rab GAP AS160 and Rab8a Rab GTPase.J Lipid Res. 53 (4): 709-17.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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