| 产品介绍 | | |
| Mouse anti Pig granulocytes antibody, clone 2B2recognizes porcine basophils, neutrophils and eosinophils, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code (MCA2599GA), allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2-early myeloid precursors, 6D10+2B2-immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins (Pérezet al.2007). |
| 产品详情 | | |
| Target Species | Pig |
| Product Form | Purified IgG conjugated to Alexa Fluor 647 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Fusion Partners | Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 mouse myeloma cell line |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Porcine bone marrow haematopoietic cells (BMHC). |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 推荐产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
| 文献 |
| 1.Pérez, C.et al.(2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.Dev Comp Immunol. 31 (3): 296-306.2.Stone, J.P.et al.(2016) Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes UsingEx VivoLung Perfusion.Am J Transplant. 16 (1): 33-43.3.Ezquerra, A.et al.(2009) Porcine myelomonocytic markers and cell populations.Dev Comp Immunol. 33 (3): 284-98.4.Gardner, D.S.et al.(2016) Remote effects of acute kidney injury in a porcine model.Am J Physiol Renal Physiol. 310 (4): F259-71.4.Nguyen, D.N.et al.(2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.Sci Rep. 6: 36816. |
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