Bio-Rad MCA2462A488 CD80 抗体 ,Rat anti mouse CD80:Alexa Fluor488-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA2462A488 CD80 抗体 ,Rat anti mouse CD80:Alexa Fluor488

2024-10-22

Rat anti mouse CD80:Alexa Fluor488

货号：MCA2462A488

规格：100 Tests/1ml

价格：3375

产品类型：流式抗体

品牌：Bio-Rad

抗原： CD80

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a

克隆号：RM80

荧光染料：Alexa Fluor 488

产品介绍
Rat anti Mouse CD80 antibody, clone RM80recognizes mouse CD80 (B7-1), a ~60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4.CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners	Spleen cells from immunised SD rats were fused with cells of the P3U1 myeloma cell line.
Buffer Solution	Phosphate buffered saline
Immunogen	Cultured LAK cells from Balb/c mice.
Approx. Protein Concentrations	IgG concentration 0.05mg/ml
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Nuriya, S.et al.(1996) The differential role of CD86 and CD80 co-stimulatory molecules in the induction and the effector phases of contact hypersensitivity.Int Immunol. 8 (6): 917-26.2.Jin LPet al.(2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.J Immunol. 173 (6): 3612-9.3.Nakajima, A.et al.(1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.Int Immunol. 9 (5): 637-44.4.Nozawa, K.et al.(2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.J Immunol. 167 (9): 4981-6.

技术参数

产品应用 Flow

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Bio-Rad MCA2462A488 CD80 抗体 ,Rat anti mouse CD80:Alexa Fluor488

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