Bio-Rad MCA2368 mouse anti human FC EPSILON R1 alpha,mouse anti human FC EPSILON R1 alpha

2024-10-22

mouse anti human FC EPSILON R1 alpha

货号:MCA2368,MCA2368T,MCA2368GA

规格:0.2 mg,25 µg,0.1 mg

价格:3740,918,2057

产品类型:标准品和抗体对

品牌:Bio-Rad

抗原:FC EPSILON R1 alpha

物种:人

宿主:小鼠

抗体亚型:IgG2b, kappa

克隆号:9E1

荧光染料:其它

产品介绍
Mouse anti Human Fc epsilon R1 alpha antibody, clone 9E1recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
Preparation
  • MCA2368GA, MCA2368: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • MCA2368T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Carrier FreeYes
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Preservative Stabilisers0.09%Sodium Azide
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/50Pack Size: 0.1 mg1/25Pack Size: 25 µg, 0.2 mg1/100Pack Size: 0.1 mg
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Vangelista, L.et al.(2002) A minimal receptor-Ig chimera of human FcepsilonRI alpha-chain efficiently binds secretory and membrane IgE.Protein Eng. 15 (1): 51-7.2.Vangelista, L.et al.(2002) Efficient folding of the FcepsilonRI alpha-chain membrane-proximal domain D2 depends on the presence of the N-terminal domain D1.J Mol Biol. 322 (4): 815-25.3.Vangelista, L.et al.(2005) Membrane IgE binds and activates Fc epsilon RI in an antigen-independent manner.J Immunol. 174 (9): 5602-11.

技术参数

产品应用 ELISA; Flow; IF;IP;WB

产品类型 单克隆抗体

推荐用于 ELISA; Flow; IF;IP;WB

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