BIO-RAD MCA2256 Rat anti Mouse CDw210a antibody, clone 1B1.3a,Rat anti Mouse CDw210a antibody, clone 1B1.3a

2024-10-22

Rat anti Mouse CDw210a antibody, clone 1B1.3a

货号:MCA2256

规格:0.25 mg

价格:3043

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CDw210a

物种:小鼠

宿主:大鼠

抗体亚型:IgG1

克隆号:1B1.3a

荧光染料:Purified

产品介绍
Rat anti Mouse CDw210a antibody, clone 1B1.3arecognizes mouse CDw210a, a cell surface glycoprotein of approximately 110 kDa, also known as Interleukin-10 receptor 1 (IL-10R1). The epitope recognized by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CDw210a. Mouse CDw210a is expressed by a range of cells including thymocytes, T cells, B cells and monocytes. Rat anti Mouse CDw210a antibody, clone 1B1.3a is reported to neutralize the effects of IL-10 (Assemanet al.1997).
产品详情
Target SpeciesMouse
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenPurified recombinant extracellular region of mouse CDw210a.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Preservative Stabilisers0.09%Sodium Azide
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Asseman, C.et al.(1999) An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.J Exp Med. 190 (7): 995-1004.2.Phillips, J.M.et al.(2001) Cutting edge: interactions through the IL-10 receptor regulate autoimmune diabetes.J Immunol. 167 (11): 6087-91.3.Sundstedt, A.et al.(2003) Role for IL-10 in suppression mediated by peptide-induced regulatory T cellsin vivo.J Immunol. 170 (3): 1240-8.4.Asseman, C.et al.(2003) Colitogenic Th1 cells are present in the antigen-experienced T cell pool in normal mice: control by CD4+ regulatory T cells and IL-10.J Immunol. 171: 971-8.
技术参数

产品应用 WB

产品类型 Monoclonal Antibody

推荐用于 WB

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