BIO-RAD MCA2086PET Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 ,Mouse anti Human CD18 (Activation E

2024-10-22

Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148

货号:MCA2086PET,MCA2086PE

规格:25 Tests,100 Tests

价格:1760,3995

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD18 (Activation Epitope)

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:MEM-148

荧光染料:PE

产品介绍
Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148recognizes an epitope on the human CD18 molecule that is hidden in the CD11/CD18 heterodimer on resting cells. Clone MEM-148 binds very weakly to resting peripheral blood leukocytes and strongly to all leukocytes upon cellular activation. The epitope recognized by Mouse anti Human CD18 (Activation Epitope) antibody, clone MEM-148 is also exposed during dissociation of the CD11/CD18 by low pH.
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
ReconstitutionPack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute in 0.25 ml disilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G
Buffer SolutionPhosphate buffered saline
Preservative StabilisersPack Size: 100 Tests<0.1% Sodium Azide (NaN3)0.05% Bovine Serum AlbuminPack Size: 25 Tests<0.1% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenPeripheral blood mononuclear cells.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee6 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Drbal, K.et al.(2001) A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells.Blood. 98 (5): 1561-6.2.Luissint, A.C.et al.(2008) JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.J Cell Biol. 183 (6): 1159-73.3.Feng, C.et al.(2011) Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1.J Leukoc Biol. 90: 313-21.

技术参数

产品应用 Flow;WB

产品类型 Monoclonal Antibody

推荐用于 Flow;WB

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