BIO-RAD MCA2025T Mouse anti Human CD49b antibody, clone 16B4,Mouse anti Human CD49b antibody, clone 16B4

2024-10-22

Mouse anti Human CD49b antibody, clone 16B4

货号:MCA2025T,MCA2025

规格:25 µg,0.2 mg

价格:935,3783

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD49b

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:16B4

荧光染料:Purified

产品介绍
Mouse anti Human CD49b antibody, clone 16B4recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multipleFG-GAPrepeats and a singleVWFAdomain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide
ImmunogenPurified human beta 1 preparation from HT1080 fibrosarcoma cell extract
Fusion PartnersSpleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Fitter, S.et al.(1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.Biochem J. 338 ( Pt 1): 61-70.2.Sincock, P.M.et al.(1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.J Cell Sci. 112 ( Pt 6): 833-44.3.Eaton, C.L.et al.(2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.Prostate. 70 (8): 875-82.4.Gassmann, P.et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.Neoplasia. 11: 651-61.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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