| 产品介绍 | | |
| Mouse anti Human CD49b antibody, clone 16B4recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multipleFG-GAPrepeats and a singleVWFAdomain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Immunogen | Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05mg/ml |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Fitter, S.et al.(1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.Biochem J. 338 ( Pt 1): 61-70.2.Sincock, P.M.et al.(1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.J Cell Sci. 112 ( Pt 6): 833-44.3.Eaton, C.L.et al.(2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.Prostate. 70 (8): 875-82.4.Gassmann, P.et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.Neoplasia. 11: 651-61. |
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