BIO-RAD MCA1972PE Mouse anti Pig CD18a, clone PNK-I,Mouse anti Pig CD18a, clone PNK-I-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA1972PE Mouse anti Pig CD18a, clone PNK-I,Mouse anti Pig CD18a, clone PNK-I

2024-10-22

Mouse anti Pig CD18a, clone PNK-I

货号：MCA1972PE

规格：100 Tests

价格：4522

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD18a

物种：pig

宿主：小鼠

抗体亚型：IgG1

克隆号：PNK-I

荧光染料：PE

产品介绍
Mouse anti Pig CD18a, clone PNK-Irecognizes porcine CD18a. PNK-I was clustered as CD18a at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmulleret al.1998). Clone PNK-I immunoprecipitates proteins of ~166 kDa, ~155 kDa and ~95 kDa under non-reducing conditions, specifically recognizing the 95 kDa protein, consistent with the integrin β2 chain (CD18). PNK-I inhibits porcine NK cell activity independent of any effect on antibody dependent cellular cytotoxicity (Dato and Kim 1990).CD18 is a single pass type I transmembrane protein and is expressed on all leukocytes and is involved in a variety of cell functions. CD18 acts as a receptor for several ICAM molecules effecting intercellular adhesion functions, it is also involved in the recognition of a variety of extracellular substrate molecules.CD18 acts as a receptor for a number of leukotoxins produced by fungi and bacteria. Clone PNK-I is able to ameliorate the effects of these leukotoxins by blocking binding of the toxins to the CD18 receptor (Chenet al.2011).
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Immunogen	Porcine large granular lymphocytes.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:RPE	MCA928PE	F	100 Tests
文献
1.Dato, M.E. & Kim, Y.B. (1990) Characterization and utilization of a monoclonal antibody inhibiting porcine natural killer cell activity for isolation of natural killer and killer cells.J Immunol. 144 (11): 4452-62.2.Haverson, K.et al. (1999) T-cell populations in the pig intestinal lamina propria: memory cells with unusual phenotypic characteristics.Immunology 96: 66-73.3.Vanden Bergh, P.G.et al. (2009) Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.Vet Res. 40:1-10.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA1972PE Mouse anti Pig CD18a, clone PNK-I,Mouse anti Pig CD18a, clone PNK-I

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