Bio-Rad MCA1940P750 CD19抗体,mouse anti human CD19:RPE-Alexa Fluor® 750

2024-10-22

mouse anti human CD19:RPE-Alexa Fluor® 750

货号:MCA1940P750

规格:100 Tests/1ml

价格:6460

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD19

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:LT19

荧光染料:其它

产品介绍
Mouse anti Human CD19 antibody, clone LT19recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rieet al.1989).CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradburyet al.1992), CD9, CD81 and CD82 (Horváthet al.1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzuttoet al.1987).
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

1% Bovine Serum Albumin

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

RPE-Alexa Fluor®750 488nm laser

496779
RPE-Alexa Fluor®750 561nm laser546779

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Tandem conjugates of RPE - Alexa Fluor®750 display fluorescence properties that are virtually identical to those of RPE - Cy7 tandem conjugates.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells or cells or 100ul whole blood.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:RPE-Alexa Fluor® 750

MCA928P750

F100 Tests/1ml
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文献
1. Girbl, T.et al.(2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.Cancer Res. 73: 561-70.2. Hertzberg, L.et al.(2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.Blood. 115: 1006-17.3. Kakko, T.et al.(2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.Diab Vasc Dis Res. 8: 221-8.4. Dorvignit, D.et al.(2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.MAbs. 4 (4): 488-96.5. Karlsen, M.et al.(2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.Scand J Immunol. 82 (6): 523-31.6. Clark, L.E.et al.(2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.Nat Commun. 9 (1): 1884.7. Gu, Y.et al.(2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.Nat Commun. 10 (1): 893.8. Yang, C.et al.(2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.PLoS One. 8 (5): e64159.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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