Bio-Rad MCA1856T CD151抗体,mouse anti human CD151-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1856T CD151抗体,mouse anti human CD151

2024-10-22

mouse anti human CD151

货号：MCA1856T ,MCA1856GA,MCA1856

规格：25 µg,0.1 mg,0.2 mg

价格：893,2023,3315

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD151

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：11G5a

荧光染料：Purified

产品介绍
Mouse anti Human CD151 antibody, clone 11G5arecognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crewet al.2004).It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch.et al.1998).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	MCA1856GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant MCA1856, MCA1856T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Flow Cytometry	√	1/50	1/100
Immunofluorescence	√
Immunohistology - Frozen	√
Immunohistology - Paraffin¹	√		1/100
Immunoprecipitation	√
Western Blotting²	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.²Clone 11G5a recognises human CD151 under non-reducing conditions.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul whole blood Histology Positive Control TissueTonsil/spleen.
数据
Staining of human peripheral blood platelets with Mouse anti Human CD151 (MCA1856)
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
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文献
1. Spoden, G.et al.(2008) Clathrin- and caveolin-independent entry of human papillomavirus type 16--involvement of tetraspanin-enriched microdomains (TEMs).PLoS One. 3: e3313.2. Huang, X.Y.et al.(2010) Overexpression of CD151 as an adverse marker for intrahepatic cholangiocarcinoma patients.Cancer. 116: 5440-51.3. Ke, A.W.et al.(2009) Role of overexpression of CD151 and/or c-Met in predicting prognosis of hepatocellular carcinoma.Hepatology. 49: 491-503.4. Devbhandari, R.P.et al.(2011) Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin β1 complex in the progression of hepatocellular carcinoma.PLoS One. 6: e24901.5. Shi, G.M.et al.(2010) CD151 modulates expression of matrix metalloproteinase 9 and promotes neoangiogenesis and progression of hepatocellular carcinoma.Hepatology. 52: 183-96.6. Nishiuchi, R.et al.(2005) Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151.Proc Natl Acad Sci U S A.102: 1939-44.7. Qiao, Y.et al.(2017) CD151, a laminin receptor showing increased expression in asthmatic patients, contributes to airway hyperresponsiveness through calcium signaling.J Allergy Clin Immunol. 139 (1): 82-92.e5.8. Hochdorfer, D.et al.(2016) Tetraspanin CD151 Promotes Initial Events in Human Cytomegalovirus Infection.J Virol. 90 (14): 6430-42.9. Wadkin, J.C.R.et al.(2017) CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma.Am J Physiol Gastrointest Liver Physiol. 313 (2): G138-G149.10. Burkard, C.et al.(2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.PLoS Pathog. 13 (2): e1006206.

技术参数

产品应用 IHC(F);ELISA; Flow; IF; IP; IHC(P) *; WB *

产品类型 Monoclonal Antibody

推荐用于 IHC(F);ELISA; Flow; IF; IP; IHC(P) *; WB *

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Bio-Rad MCA1856T CD151抗体,mouse anti human CD151

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