| 产品介绍 | | |
| Mouse anti Human CD163 antibody, clone EDHu-1recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsenet al.2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich,SRCR domainsthe third domain being critical as, cleavage of this domain at the major cleavage siteASP-265abrogates binding to the N-terminal fragment. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rhesus Monkey | √ |
| Sheep | √ |
| Pig | √ |
| Guinea Pig | √ |
| Bovine | √ |
| Cynomolgus monkey | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from ligh |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | | |
| Immunoprecipitation | √ | | |
| Functional Assays | | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928AF488 | Flow | 100 Tests |
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| 文献 |
| 1. Frafjord, A.et al.(2020) Antibody combinations for optimized staining of macrophages in human lung tumours.Scand J Immunol. 92 (1): e12889.2. Ma, H.et al.(2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndromevirus-2 infectionin vitro..Vet Res. 52 (1): 97.3. Kemmerer, C.L.et al.(2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patientsBMC Cancer. 21(1):1108.4. Lehmann, M.et al.(2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.J Eur Acad Dermatol Venereol. 35 (11): e752-e755.5. Baumann, D.et al.(2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.J Immunother Cancer.9 (7): e002319.6. Ikezumi, Y.et al.(2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.Pediatr Nephrol. 36 (2): 349-59.7. Eligini, S.et al.(2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.Sci Rep. 9 (1): 8680.8. Edwards, J.H.et al.(2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.Biomaterials. 279: 121204.9. Ströbel, S.et al.(2021) A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.Sci Rep. 11 (1): 22765.10. Galea, I.et al.(2022) Iron Deposition in the Brain After Aneurysmal Subarachnoid Hemorrhage.Stroke. 53 (5): 1633-42. |
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